Acknowledgments	5
	Contents	12
	Table of Contents	12
	Section III Supporting Methodologies	15
	3: Getting Started with Tissue Culture: Media Preparation, Sterile Technique, and Laboratory Equipment	16
	4: Histological Techniques	39
	5: Photographic Methods for Plant Cell and Tissue Culture	51
	6: Elements of In Vitro Research	58
	7: A Brief Introduction to Plant Anatomy	79
	8: Plant Growth Regulators in Plant Tissue Culture and Development	99
	9: Software and Databases as Tools for Analyzing Nucleic Acid and Protein Sequences*	116
	10: Molecular Approaches to the Study of Plant Development	136
	Section IV Propagation and Development Concepts	169
	11: Shoot Culture Procedures	170
	12: Propagation from Nonmeristematic Tissues: Organogenesis	187
	13: Molecular Aspects of In Vitro Shoot Organogenesis	206
	14: Propagation from Nonmeristematic Tissues: Nonzygotic Embryogenesis*	223
	15: Some Developmental and Molecular Aspects of Somatic Embryogenesis (Nonzygotic Embryogenesis)	243
	Section V Crop Improvement Techniques	254
	16: Use of Protoplasts for Plant Improvement	255
	18: Embryo Rescue	1
	19: Genetic Engineering Technologies*	289
	20: Genetically Modified Plant Controversies: Sensational Headlines versus Sensible Research	301
	21: Construction and Use of a Simple Gene Gun for Particle Bombardment*	317
	22: A Simple Illumination System for Visualizing Green Fluorescent Protein	327
	23: Germplasm Preservation	333
	24: Valuable Secondary Products from In Vitro	344
	Section VI Special Topics	362
	25: In Vitro Plant Pathology*	1
	26: Variation in Tissue Culture*	363
	27: Commercial Laboratory Production	1
	28: Indexing for Plant Pathogens	376
	29: Entrepreneurship for Biotechnology Ventures: From Bench to Bag	391
	Index	402