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Regulation of Gene Expression
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Regulation of Gene Expression
von: Gary H. Perdew, Jack P. Vanden Heuvel, Jeffrey M. Peters
Humana Press, 2008
ISBN: 9781597452281
333 Seiten, Download: 6471 KB
 
Format:  PDF
geeignet für: Apple iPad, Android Tablet PC's Online-Lesen PC, MAC, Laptop

Typ: B (paralleler Zugriff)

 

 
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Inhaltsverzeichnis

  Preface 5  
  Contents 7  
  COMPANION CD 9  
  Part I GENE EXPRESSION CONTROL AT THE MRNA LEVEL 10  
     Messenger RNA Accumulation 20  
        1. Concepts 20  
        2. Basic Methods and Approaches 23  
        3. Summary 38  
     Transcript Profiling 40  
        1. Concepts 40  
        2. Methods and Approaches 42  
        3. Summary 57  
     Transcriptional Regulation of Gene Expression 58  
        1. Concepts 58  
        2. Methods and Approaches 69  
        3. Summary 107  
     Posttranscriptional Processing of Messenger RNA 114  
        1. Concepts 114  
        2. Methods and Approaches 125  
        3. Summary 145  
     Study Questions 150  
     References 152  
  Part II REGULATION OF PROTEIN LEVELS AND TRANSCRIPTION FACTOR FUNCTION 156  
     Overview 158  
     Protein Synthesis and Turnover 164  
        1. Concepts 164  
        2. Methods and Approaches 166  
        3. Summary 168  
     Proteomics 170  
        1. Concepts 170  
        2. Methods and Approaches 171  
        3. Summary 181  
     Determination of Protein–Protein Interactions and the Motifs That Mediate Them 182  
        1. Concepts 182  
        2. Methods and Approaches 182  
        3. Summary 223  
     Posttranslational Modifications 224  
        1. Concepts 224  
        2. Methods and Approaches 229  
        3. Summary 246  
     Dissection of Signaling Pathways 248  
        1. Concepts 248  
        2. Methods and Approaches 249  
        3. Summary 266  
     Study Questions 268  
     References 270  
  Part III USE OF TRANSGENIC AND KNOCKOUT MICE TO STUDY GENE REGULATION 274  
     OVERVIEW 276  
     Production of Transgenic Animals 280  
        1. Standard Production 280  
        2. Applications of Transgenic Models 287  
     KNOCKOUT ANIMALS 304  
        1. Standard Production 304  
        2. Applications of Null Mouse Models 309  
     Isolation of Cells and Cell Lines From Transgenic and Knockout Animals 328  
        1. Standard Production 328  
     Study Questions 330  
     References 332  
  Index 338  


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