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Preface |
5 |
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Contents |
7 |
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COMPANION CD |
9 |
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Part I GENE EXPRESSION CONTROL AT THE MRNA LEVEL |
10 |
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Messenger RNA Accumulation |
20 |
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1. Concepts |
20 |
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2. Basic Methods and Approaches |
23 |
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3. Summary |
38 |
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|
Transcript Profiling |
40 |
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1. Concepts |
40 |
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2. Methods and Approaches |
42 |
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3. Summary |
57 |
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|
Transcriptional Regulation of Gene Expression |
58 |
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1. Concepts |
58 |
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2. Methods and Approaches |
69 |
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3. Summary |
107 |
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Posttranscriptional Processing of Messenger RNA |
114 |
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1. Concepts |
114 |
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2. Methods and Approaches |
125 |
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3. Summary |
145 |
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Study Questions |
150 |
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References |
152 |
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Part II REGULATION OF PROTEIN LEVELS AND TRANSCRIPTION FACTOR FUNCTION |
156 |
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Overview |
158 |
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Protein Synthesis and Turnover |
164 |
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1. Concepts |
164 |
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2. Methods and Approaches |
166 |
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3. Summary |
168 |
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Proteomics |
170 |
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1. Concepts |
170 |
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2. Methods and Approaches |
171 |
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3. Summary |
181 |
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Determination of Protein–Protein Interactions and the Motifs That Mediate Them |
182 |
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1. Concepts |
182 |
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2. Methods and Approaches |
182 |
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3. Summary |
223 |
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Posttranslational Modifications |
224 |
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1. Concepts |
224 |
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2. Methods and Approaches |
229 |
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3. Summary |
246 |
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Dissection of Signaling Pathways |
248 |
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1. Concepts |
248 |
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2. Methods and Approaches |
249 |
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3. Summary |
266 |
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Study Questions |
268 |
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References |
270 |
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Part III USE OF TRANSGENIC AND KNOCKOUT MICE TO STUDY GENE REGULATION |
274 |
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OVERVIEW |
276 |
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Production of Transgenic Animals |
280 |
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1. Standard Production |
280 |
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2. Applications of Transgenic Models |
287 |
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KNOCKOUT ANIMALS |
304 |
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1. Standard Production |
304 |
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2. Applications of Null Mouse Models |
309 |
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Isolation of Cells and Cell Lines From Transgenic and Knockout Animals |
328 |
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1. Standard Production |
328 |
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Study Questions |
330 |
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References |
332 |
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|
Index |
338 |
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