| |
Contents |
6 |
|
| |
Preface |
10 |
|
| |
Abbreviations |
11 |
|
| |
1 An overview of gene targeting and stem cells |
14 |
|
| |
2 Advances in gene targeting in murine embryonic stem cells |
23 |
|
| |
3 Nuclear transfer with murine embryonic stem cells |
35 |
|
| |
4 Gene targeting in sheep |
50 |
|
| |
5 RNA interference |
72 |
|
| |
6 Human embryonal carcinoma cells: surrogate tools in the study of human embryonic stem cells |
98 |
|
| |
7 Isolation of human embryonic stem cells |
122 |
|
| |
8 In vitro culture and differentiation of human embryonic stem cells |
134 |
|
| |
9 Transfection of human embryonic stem cells |
156 |
|
| |
Index |
177 |
|
