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Acknowledgments |
5 |
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Contents |
12 |
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Table of Contents |
12 |
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Section III Supporting Methodologies |
15 |
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3: Getting Started with Tissue Culture: Media Preparation, Sterile Technique, and Laboratory Equipment |
16 |
|
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4: Histological Techniques |
39 |
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5: Photographic Methods for Plant Cell and Tissue Culture |
51 |
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6: Elements of In Vitro Research |
58 |
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7: A Brief Introduction to Plant Anatomy |
79 |
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8: Plant Growth Regulators in Plant Tissue Culture and Development |
99 |
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9: Software and Databases as Tools for Analyzing Nucleic Acid and Protein Sequences* |
116 |
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10: Molecular Approaches to the Study of Plant Development |
136 |
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Section IV Propagation and Development Concepts |
169 |
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11: Shoot Culture Procedures |
170 |
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12: Propagation from Nonmeristematic Tissues: Organogenesis |
187 |
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13: Molecular Aspects of In Vitro Shoot Organogenesis |
206 |
|
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14: Propagation from Nonmeristematic Tissues: Nonzygotic Embryogenesis* |
223 |
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15: Some Developmental and Molecular Aspects of Somatic Embryogenesis (Nonzygotic Embryogenesis) |
243 |
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Section V Crop Improvement Techniques |
254 |
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16: Use of Protoplasts for Plant Improvement |
255 |
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18: Embryo Rescue |
1 |
|
|
19: Genetic Engineering Technologies* |
289 |
|
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20: Genetically Modified Plant Controversies: Sensational Headlines versus Sensible Research |
301 |
|
|
21: Construction and Use of a Simple Gene Gun for Particle Bombardment* |
317 |
|
|
22: A Simple Illumination System for Visualizing Green Fluorescent Protein |
327 |
|
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23: Germplasm Preservation |
333 |
|
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24: Valuable Secondary Products from In Vitro |
344 |
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Section VI Special Topics |
362 |
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25: In Vitro Plant Pathology* |
1 |
|
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26: Variation in Tissue Culture* |
363 |
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27: Commercial Laboratory Production |
1 |
|
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28: Indexing for Plant Pathogens |
376 |
|
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29: Entrepreneurship for Biotechnology Ventures: From Bench to Bag |
391 |
|
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Index |
402 |
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